cfx384 real time system (Bio-Rad)
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cfx384 real time system
Cfx384 Real Time System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx384+real+time+pcr+detection+system/CFX384+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm42844465-758-5-8
Average 99 stars, based on 9947 article reviews
Cfx384 Real Time System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx384+real+time+pcr+detection+system/CFX384+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm42844465-758-5-8
Average 99 stars, based on 9947 article reviews
cfx384 real time system - by Bioz Stars,
2026-10
99/100 stars
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Quantitative RT-PCR:Article Title: Probiotics Alleviate Nonylphenol-Induced Hepatotoxicity in Silurus meridionalis via Reprogramming Arachidonic Acid Metabolism and Suppressing Ferroptosis: A Preliminary Study Article Snippet: Primers were synthesized by Sangon Biotech (Shanghai, China). .. RT-qPCR assays were performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a Article Title: Separating food intake-dependent and -independent effects in cancer cachexia Article Snippet: One microgram of purified RNA was converted to cDNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, #1708840). .. The diluted cDNA was used for RT-qPCR using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725270) with the SYBR Green Assay:Article Title: Probiotics Alleviate Nonylphenol-Induced Hepatotoxicity in Silurus meridionalis via Reprogramming Arachidonic Acid Metabolism and Suppressing Ferroptosis: A Preliminary Study Article Snippet: Primers were synthesized by Sangon Biotech (Shanghai, China). .. RT-qPCR assays were performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a Article Title: BAMBI modulates proliferation, apoptosis, and hormone secretion in Jinding duck granulosa cells. Article Snippet: The BMP and activin membrane-bound inhibitor (BAMBI) gene is a pseudoreceptor involved in TGF-β signaling and plays an important role in follicular development.. This study investigated the regulatory effects of BAMBI on the proliferation, apoptosis, and hormone secretion of follicular granulosa cells (GCs) in Jinding duck (Anas platyrhynchos domesticus).. The recombinant plasmid duck BAMBI–pcDNA3.1 × 3Flag was successfully constructed, and both interference and overexpression systems were effectively expressed in GCs.EdU incorporation assays demonstrated that BAMBI interference significantly enhanced GC proliferation and upregulated the proliferating cell nuclear antigen (PCNA) expression, whereas BAMBI overexpression markedly inhibited proliferation and reduced PCNA mRNA and protein levels. Article Title: Separating food intake-dependent and -independent effects in cancer cachexia Article Snippet: One microgram of purified RNA was converted to cDNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, #1708840). .. The diluted cDNA was used for RT-qPCR using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725270) with the Article Title: The E3 ubiquitin ligase CsPUB26 promotes citrus disease resistance by targeting the negative regulator CsWRKY57 for ubiquitination and degradation Article Snippet: Postharvest green mold, caused by Penicillium digitatum (P. digitatum), represented an important source of citrus fruit loss during storage.. Although the ubiquitin–proteasome system had been widely linked to plant immune regulation, its involvement in postharvest fruit resistance had not yet been clearly defined.. In this study, the Ubox-type E3 ubiquitin ligase, CsPUB26, was identified as a positive regulator of citrus resistance to P. digitatum infection. Article Title: Early-life combined supplementation with lactoferrin and Bifidobacterium breve HH079 provides enhanced protection against Citrobacter rodentium infection in neonatal mice Article Snippet: Briefly, total DNA was extracted from mouse fecal samples using QIAamp DNA Mini Kit (Qiagen, Germany), and DNA concentration was evaluated with a NanoDrop spectrophotometer. .. Quantitative PCR was performed on a Article Title: Indoor relative humidity and air mycobiome in eastern Taiwan residences: associations with Aspergillus and Wallemia Article Snippet: The indoor air mycobiome comprises a complex mixture of fungi, some of which are allergenic, and is associated with outdoor and indoor environmental factors, including the surrounding landscape, meteorological factors, ventilation, and indoor dampness.. However, previous studies, primarily culture-based, have reported inconsistent associations between indoor airborne fungi and indoor relative humidity (RH), with limited quantification at the genus level.. Therefore, a cross-sectional study was conducted in 198 residences in Hualien County, an eastern subtropical region of Taiwan, to examine associations between indoor RH and airborne abundance of fungal genera. Polymerase Chain Reaction:Article Title: Probiotics Alleviate Nonylphenol-Induced Hepatotoxicity in Silurus meridionalis via Reprogramming Arachidonic Acid Metabolism and Suppressing Ferroptosis: A Preliminary Study Article Snippet: Primers were synthesized by Sangon Biotech (Shanghai, China). .. RT-qPCR assays were performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a Real-time Polymerase Chain Reaction:Article Title: Probiotics Alleviate Nonylphenol-Induced Hepatotoxicity in Silurus meridionalis via Reprogramming Arachidonic Acid Metabolism and Suppressing Ferroptosis: A Preliminary Study Article Snippet: Primers were synthesized by Sangon Biotech (Shanghai, China). .. RT-qPCR assays were performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a Article Title: BAMBI modulates proliferation, apoptosis, and hormone secretion in Jinding duck granulosa cells. Article Snippet: The BMP and activin membrane-bound inhibitor (BAMBI) gene is a pseudoreceptor involved in TGF-β signaling and plays an important role in follicular development.. This study investigated the regulatory effects of BAMBI on the proliferation, apoptosis, and hormone secretion of follicular granulosa cells (GCs) in Jinding duck (Anas platyrhynchos domesticus).. The recombinant plasmid duck BAMBI–pcDNA3.1 × 3Flag was successfully constructed, and both interference and overexpression systems were effectively expressed in GCs.EdU incorporation assays demonstrated that BAMBI interference significantly enhanced GC proliferation and upregulated the proliferating cell nuclear antigen (PCNA) expression, whereas BAMBI overexpression markedly inhibited proliferation and reduced PCNA mRNA and protein levels. Article Title: Separating food intake-dependent and -independent effects in cancer cachexia Article Snippet: One microgram of purified RNA was converted to cDNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, #1708840). .. The diluted cDNA was used for RT-qPCR using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, #1725270) with the Article Title: The E3 ubiquitin ligase CsPUB26 promotes citrus disease resistance by targeting the negative regulator CsWRKY57 for ubiquitination and degradation Article Snippet: Postharvest green mold, caused by Penicillium digitatum (P. digitatum), represented an important source of citrus fruit loss during storage.. Although the ubiquitin–proteasome system had been widely linked to plant immune regulation, its involvement in postharvest fruit resistance had not yet been clearly defined.. In this study, the Ubox-type E3 ubiquitin ligase, CsPUB26, was identified as a positive regulator of citrus resistance to P. digitatum infection. Article Title: Early-life combined supplementation with lactoferrin and Bifidobacterium breve HH079 provides enhanced protection against Citrobacter rodentium infection in neonatal mice Article Snippet: Briefly, total DNA was extracted from mouse fecal samples using QIAamp DNA Mini Kit (Qiagen, Germany), and DNA concentration was evaluated with a NanoDrop spectrophotometer. .. Quantitative PCR was performed on a Article Title: Indoor relative humidity and air mycobiome in eastern Taiwan residences: associations with Aspergillus and Wallemia Article Snippet: The indoor air mycobiome comprises a complex mixture of fungi, some of which are allergenic, and is associated with outdoor and indoor environmental factors, including the surrounding landscape, meteorological factors, ventilation, and indoor dampness.. However, previous studies, primarily culture-based, have reported inconsistent associations between indoor airborne fungi and indoor relative humidity (RH), with limited quantification at the genus level.. Therefore, a cross-sectional study was conducted in 198 residences in Hualien County, an eastern subtropical region of Taiwan, to examine associations between indoor RH and airborne abundance of fungal genera. Article Title: Transposable Element Dynamics Drive the Genomic Evolution and Phenotypic Diversification of Allotetraploid Common Carp. Article Snippet: TRIzol (Invitrogen, China) reagent was used to extract total RNA from the collected skin tissues, and reverse transcription was carried out with the Hifair III First Strand cDNA Synthesis Kit (gDNA Digester Plus, Yeasen, China) following the manufacturer’s instructions. .. Then, quantitative real-time PCR (qRT-PCR) was performed with the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, China) on a Article Title: Dietary oil composition modulates metabolic endotoxemia in an in vitro human intestinal-immune cell model Article Snippet: Subsequently, 500 ng of RNA was used to synthesize cDNA using the iScript cDNA synthesis Kit (Bio-Rad Laboratories, Veenendaal, the Netherlands), following the manufacturer's instructions. .. Gene expression was determined by real-time qPCR using SensiMix SYBR No-ROX Kit (Bioline, GC Biotech, Waddinxveen, the Netherlands) on a Gene Expression:Article Title: The E3 ubiquitin ligase CsPUB26 promotes citrus disease resistance by targeting the negative regulator CsWRKY57 for ubiquitination and degradation Article Snippet: Postharvest green mold, caused by Penicillium digitatum (P. digitatum), represented an important source of citrus fruit loss during storage.. Although the ubiquitin–proteasome system had been widely linked to plant immune regulation, its involvement in postharvest fruit resistance had not yet been clearly defined.. In this study, the Ubox-type E3 ubiquitin ligase, CsPUB26, was identified as a positive regulator of citrus resistance to P. digitatum infection. Article Title: Dietary oil composition modulates metabolic endotoxemia in an in vitro human intestinal-immune cell model Article Snippet: Subsequently, 500 ng of RNA was used to synthesize cDNA using the iScript cDNA synthesis Kit (Bio-Rad Laboratories, Veenendaal, the Netherlands), following the manufacturer's instructions. .. Gene expression was determined by real-time qPCR using SensiMix SYBR No-ROX Kit (Bioline, GC Biotech, Waddinxveen, the Netherlands) on a Plasmid Preparation:Article Title: Indoor relative humidity and air mycobiome in eastern Taiwan residences: associations with Aspergillus and Wallemia Article Snippet: The indoor air mycobiome comprises a complex mixture of fungi, some of which are allergenic, and is associated with outdoor and indoor environmental factors, including the surrounding landscape, meteorological factors, ventilation, and indoor dampness.. However, previous studies, primarily culture-based, have reported inconsistent associations between indoor airborne fungi and indoor relative humidity (RH), with limited quantification at the genus level.. Therefore, a cross-sectional study was conducted in 198 residences in Hualien County, an eastern subtropical region of Taiwan, to examine associations between indoor RH and airborne abundance of fungal genera. Construct:Article Title: Indoor relative humidity and air mycobiome in eastern Taiwan residences: associations with Aspergillus and Wallemia Article Snippet: The indoor air mycobiome comprises a complex mixture of fungi, some of which are allergenic, and is associated with outdoor and indoor environmental factors, including the surrounding landscape, meteorological factors, ventilation, and indoor dampness.. However, previous studies, primarily culture-based, have reported inconsistent associations between indoor airborne fungi and indoor relative humidity (RH), with limited quantification at the genus level.. Therefore, a cross-sectional study was conducted in 198 residences in Hualien County, an eastern subtropical region of Taiwan, to examine associations between indoor RH and airborne abundance of fungal genera. Sequencing:Article Title: Indoor relative humidity and air mycobiome in eastern Taiwan residences: associations with Aspergillus and Wallemia Article Snippet: The indoor air mycobiome comprises a complex mixture of fungi, some of which are allergenic, and is associated with outdoor and indoor environmental factors, including the surrounding landscape, meteorological factors, ventilation, and indoor dampness.. However, previous studies, primarily culture-based, have reported inconsistent associations between indoor airborne fungi and indoor relative humidity (RH), with limited quantification at the genus level.. Therefore, a cross-sectional study was conducted in 198 residences in Hualien County, an eastern subtropical region of Taiwan, to examine associations between indoor RH and airborne abundance of fungal genera. Qubit:Article Title: Indoor relative humidity and air mycobiome in eastern Taiwan residences: associations with Aspergillus and Wallemia Article Snippet: The indoor air mycobiome comprises a complex mixture of fungi, some of which are allergenic, and is associated with outdoor and indoor environmental factors, including the surrounding landscape, meteorological factors, ventilation, and indoor dampness.. However, previous studies, primarily culture-based, have reported inconsistent associations between indoor airborne fungi and indoor relative humidity (RH), with limited quantification at the genus level.. Therefore, a cross-sectional study was conducted in 198 residences in Hualien County, an eastern subtropical region of Taiwan, to examine associations between indoor RH and airborne abundance of fungal genera. |